Evaluation of Dynamic Thiol-Disulphide Homeostasis in Patients with Epilepsy

Abstract

Objectives:The aim of this study was to investigate dynamic thiol-disulphide homeostasis as a novel oxidative stress parameter in patients with epilepsy.Methods:A total of 100 participants were included in the study. Of these, 50 participants were diagnosed with epilepsy and remaining 50 were healthy individuals. Total thiol (–SH+–S-S–) and native thiol (–SH) levels in serum were measured in all patients. Quantity of dynamic disulphide bond and (–S-S–) x 100 / (-SH), (–S-S-) x 100 / (–SH+–S-S–), and –SH x100 / (–SH+–S-S–) ratios were calculated from these values. Data obtained were compared between patients with epilepsy and healthy individuals.Results:No statically significant difference was determined between patients with epilepsy and healthy individuals in terms of total thiol, native thiol, and dynamic disulphide bond levels and (–S-S–) x 100 / (-SH), (–S-S-) x 100 / (–SH+–S-S–), and –SH x 100 / (–SH+–S-S–) ratios. Neither was there significant correlation between total thiol, native thiol, and dynamic disulphide bond levels and (–S-S–), (–S-S–) x 100 / (-SH), (–S-S-) x 100 / (–SH+–S-S–) and –SH x 100 / (–SH+–S-S–) ratios of patients and seizure frequency or duration of illness.Conclusion:Oxidative stress is considered to be one of the molecular changes that are the underlying causes of epileptogenesis. In this study, we investigated dynamic thiol-disulfide homeostasis in patients with epilepsy using a new method in the literature.

Keywords

• dynamic thiol-disulphide homeostasis
• epilepsy
• oxidative stress
• thiol metabolism