Development of genic and genomic microsatellites in Gleditsia triacanthos L. (Fabaceae) using Illumina sequencing

Yawen Wu; Ruhua Zhang; Margaret Staton; Scott E. Schlarbaum; Mark V. Coggeshall; Jeanne Romero-Severson; John E. Carlson; Haiying Liang; Yi Xu; Daniela I. Drautz-Moses; Stephan C. Schuster; Oliver Gailing

doi:10.15287/afr.2017.819

Annals of Forest Research (Dec 2017)

Development of genic and genomic microsatellites in Gleditsia triacanthos L. (Fabaceae) using Illumina sequencing

Yawen Wu,
Ruhua Zhang,
Margaret Staton,
Scott E. Schlarbaum,
Mark V. Coggeshall,
Jeanne Romero-Severson,
John E. Carlson,
Haiying Liang,
Yi Xu,
Daniela I. Drautz-Moses,
Stephan C. Schuster,
Oliver Gailing

Affiliations

Yawen Wu: Michigan Technological University, School of Forest Resources and Environmental Science, 1400 Townsend Drive, Houghton, MI 49931
Ruhua Zhang: Michigan Technological University, School of Forest Resources and Environmental Science, 1400 Townsend Drive, Houghton, MI 49931, USA
Margaret Staton: Department of Entomology and Plant Pathology, The University of Tennessee, Knoxville, TN 37996, USA
Scott E. Schlarbaum: Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-4563, USA
Mark V. Coggeshall: USDA Forest Service, Northern Research Station, Purdue University, West Lafayette, IN 47907, USA
Jeanne Romero-Severson: Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
John E. Carlson: The Department of Ecosystem Science and Management and Department of Plant Science, Pennsylvania State University, University Park, PA 16802, SUA
Haiying Liang: Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
Yi Xu: Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ 08901, USA
Daniela I. Drautz-Moses: The Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
Stephan C. Schuster: The Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
Oliver Gailing: University of Göttingen, Forest Genetics and Forest Tree Breeding, Büsgenweg 2, 37077 Göttingen, Germany

DOI: https://doi.org/10.15287/afr.2017.819
Journal volume & issue: Vol. 60, no. 2
pp. 343 – 350

Abstract

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Twenty new polymorphic genic SSRs (EST-SSRs) and 13 genomic SSRs were developed in honeylocust (Gleditsia triacanthos) using Illumina transcriptome and low-coverage genome sequencing. A diversity panel of 40 honeylocust samples covering large parts of the species distribution range was characterized. As expected the level of genetic variation was lower in EST-SSRs than for non-genic genomic SSRs. This is the first report of EST-SSRs for honeylocust. All markers are polymorphic and produce clear single locus amplification products and can be used for genetic diversity and gene flow analyses. The transcriptome sequencing data provide a rich resource for new marker development.

est-ssrs, transcriptome, gleditsia triacanthos, next-generation sequencing

Published in Annals of Forest Research

ISSN: 1844-8135 (Print); 2065-2445 (Online)
Publisher: ‘Marin Drăcea’ National Research-Development Institute in Forestry
Country of publisher: Romania
LCC subjects: Agriculture: Forestry
Website: http://www.afrjournal.org

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