Genome-Wide Characterization and Analysis of R2R3-MYB Genes Related to Fruit Ripening and Stress Response in Banana (<i>Musa acuminata</i> L. AAA Group, cv. ‘Cavendish’)
Zhuo Wang,
Xiaoming Yao,
Caihong Jia,
Yunke Zheng,
Qiumei Lin,
Jingyi Wang,
Juhua Liu,
Zhao Zhu,
Long Peng,
Biyu Xu,
Xinli Cong,
Zhiqiang Jin
Affiliations
Zhuo Wang
Key Laboratory of Tropical Crop Biotechnology of Ministry of Agriculture and Rural Affairs of China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Xiaoming Yao
BGI-Sanya, BGI-Shenzhen, Sanya 572025, China
Caihong Jia
Key Laboratory of Tropical Crop Biotechnology of Ministry of Agriculture and Rural Affairs of China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Yunke Zheng
Key Laboratory of Tropical Crop Biotechnology of Ministry of Agriculture and Rural Affairs of China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Qiumei Lin
Key Laboratory of Tropical Crop Biotechnology of Ministry of Agriculture and Rural Affairs of China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Jingyi Wang
Key Laboratory of Tropical Crop Biotechnology of Ministry of Agriculture and Rural Affairs of China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Juhua Liu
Key Laboratory of Tropical Crop Biotechnology of Ministry of Agriculture and Rural Affairs of China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Zhao Zhu
College of Tropical Crops, Yunnan Agricultural University, Pu’er 665000, China
Long Peng
College of Tropical Crops, Yunnan Agricultural University, Pu’er 665000, China
Biyu Xu
Key Laboratory of Tropical Crop Biotechnology of Ministry of Agriculture and Rural Affairs of China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Xinli Cong
School of Life Sciences, Hainan University, Haikou 570228, China
Zhiqiang Jin
Key Laboratory of Tropical Crop Biotechnology of Ministry of Agriculture and Rural Affairs of China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
MYB is an important type of transcription factor in eukaryotes. It is widely involved in a variety of biological processes and plays a role in plant morphogenesis, growth and development, primary and secondary metabolite synthesis, and other life processes. In this study, bioinformatics methods were used to identify the R2R3-MYB transcription factor family members in the whole Musa acuminata (DH-Pahang) genome, one of the wild ancestors of banana. A total of 280 MaMYBs were obtained, and phylogenetic analysis indicated that these MaMYBs could be classified into 33 clades with MYBs from Arabidopsis thaliana. The amino acid sequences of the R2 and R3 Myb-DNA binding in all MaMYB protein sequences were quite conserved, especially Arg-12, Arg-13, Leu-23, and Leu-79. Distribution mapping results showed that 277 MaMYBs were localized on the 11 chromosomes in the Musa acuminata genome. The MaMYBs were distributed unevenly across the 11 chromosomes. More than 40.0% of the MaMYBs were located in collinear fragments, and segmental duplications likely played a key role in the expansion of the MaMYBs. Moreover, the expression profiles of MaMYBs in different fruit development and ripening stages and under various abiotic and biotic stresses were investigated using available RNA-sequencing data to obtain fruit development, ripening-specific, and stress-responsive candidate genes. Weighted gene co-expression network analysis (WGCNA) was used to analyze transcriptome data of banana from the above 11 samples. We found MaMYBs participating in important metabolic biosynthesis pathways in banana. Collectively, our results represent a comprehensive genome-wide study of the MaMYB gene family, which should be helpful in further detailed studies on MaMYBs functions related to fruit development, postharvest ripening, and the seedling response to stress in an important banana cultivar.
