Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1

Abstract

Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.

Keywords

• bovine herpesvirus-1
• cosolvents
• polymerase chain reaction