Comparative Effect of Three Different Exercise Intensities in Combination with Diazoxide on Contraction Capacity and Oxidative Stress of Skeletal Muscle in Obese Rats
Mariana Gómez-Barroso,
Manuel A. Vargas-Vargas,
Donovan J. Peña-Montes,
Christian Cortés-Rojo,
Alfredo Saavedra-Molina,
Elizabeth Sánchez-Duarte,
Alain R. Rodríguez-Orozco,
Rocío Montoya-Pérez
Affiliations
Mariana Gómez-Barroso
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia 58030, Mexico
Manuel A. Vargas-Vargas
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia 58030, Mexico
Donovan J. Peña-Montes
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia 58030, Mexico
Christian Cortés-Rojo
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia 58030, Mexico
Alfredo Saavedra-Molina
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia 58030, Mexico
Elizabeth Sánchez-Duarte
Departamento de Ciencias Aplicadas al Trabajo, Universidad de Guanajuato, Campus León, Eugenio Garza Sada 572, Lomas del Campestre Sección 2, León 37150, Mexico
Alain R. Rodríguez-Orozco
Facultad de Ciencias Médicas y Biológicas “Dr. Ignacio Chavez”, Universidad Michoacana de San Nicolás de Hidalgo Av. Dr. Rafael Carrillo S/N Esq. Dr. Salvador González Herrejon, Bosque Cuauhtémoc, Morelia 58020, Mexico
Rocío Montoya-Pérez
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Col. Felicitas del Río, Morelia 58030, Mexico
Obesity is a chronic disease that impairs skeletal muscle function, affects the ability to contract, and promotes the development of fatigue. For this reason, the study of treatments that seek to reduce the harmful effects of obesity on muscle tissue has been deepened. Diazoxide treatment and various exercise protocols have been proposed to protect skeletal muscle against oxidative stress and its effects. However, the intensity and duration of exercise combined with diazoxide that would obtain the best results for improving skeletal muscle function in obese rats is unknown. To this end, this study evaluated the effects of three different exercise intensities combined with diazoxide on contraction capacity, resistance to fatigue, markers of oxidative stress, lipid peroxidation, ROS, and glutathione redox status of skeletal muscle. The results showed that treatments with diazoxide and exercise at different intensities improved muscle contraction capacity by reducing oxidative stress during obesity, with the best results being obtained with low-intensity exercise in combination with diazoxide. Therefore, these results suggest that diazoxide and low-intensity exercise improve muscle function during obesity by decreasing oxidative stress with the same efficiency as a moderate-intensity exercise protocol.
