PLoS Pathogens (Dec 2020)

Assembly of infectious enteroviruses depends on multiple, conserved genomic RNA-coat protein contacts.

  • Rebecca Chandler-Bostock,
  • Carlos P Mata,
  • Richard J Bingham,
  • Eric C Dykeman,
  • Bo Meng,
  • Tobias J Tuthill,
  • David J Rowlands,
  • Neil A Ranson,
  • Reidun Twarock,
  • Peter G Stockley

DOI
https://doi.org/10.1371/journal.ppat.1009146
Journal volume & issue
Vol. 16, no. 12
p. e1009146

Abstract

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Picornaviruses are important viral pathogens, but despite extensive study, the assembly process of their infectious virions is still incompletely understood, preventing the development of anti-viral strategies targeting this essential part of the life cycle. We report the identification, via RNA SELEX and bioinformatics, of multiple RNA sites across the genome of a typical enterovirus, enterovirus-E (EV-E), that each have affinity for the cognate viral capsid protein (CP) capsomer. Many of these sites are evolutionarily conserved across known EV-E variants, suggesting they play essential functional roles. Cryo-electron microscopy was used to reconstruct the EV-E particle at ~2.2 Å resolution, revealing extensive density for the genomic RNA. Relaxing the imposed symmetry within the reconstructed particles reveals multiple RNA-CP contacts, a first for any picornavirus. Conservative mutagenesis of the individual RNA-contacting amino acid side chains in EV-E, many of which are conserved across the enterovirus family including poliovirus, is lethal but does not interfere with replication or translation. Anti-EV-E and anti-poliovirus aptamers share sequence similarities with sites distributed across the poliovirus genome. These data are consistent with the hypothesis that these RNA-CP contacts are RNA Packaging Signals (PSs) that play vital roles in assembly and suggest that the RNA PSs are evolutionarily conserved between pathogens within the family, augmenting the current protein-only assembly paradigm for this family of viruses.