The Potential Utility of Circulating Oncofetal H19 Derived miR-675 Expression versus Tissue lncRNA-H19 Expression in Diagnosis and Prognosis of HCC in Egyptian Patients
Shimaa Abdelsattar,
Dina Sweed,
Hala F. M. Kamel,
Zeinab A. Kasemy,
Abdallah M. Gameel,
Hassan Elzohry,
Omnia Ameen,
Eman Ibrahim Elgizawy,
Ahmed Sallam,
Asmaa Mosbeh,
Mahmoud S. Abdallah,
Fatma O. Khalil,
Hiba S. Al-Amodi,
Sally M. El-Hefnway
Affiliations
Shimaa Abdelsattar
Clinical Biochemistry and Molecular Diagnostics Department, National Liver Institute, Menofia University, Shebin El-Kom 32511, Egypt
Dina Sweed
Pathology Department, National Liver Institute, Menofia University, Shebin El-Kom 32511, Egypt
Hala F. M. Kamel
Department of Biochemistry, Faculty of Medicine, Umm Al Qura University, Makka 21955, Saudi Arabia
Zeinab A. Kasemy
Public Health and Community Medicine Department, Faculty of Medicine, Menofia University, Shebin El-Kom 32511, Egypt
Abdallah M. Gameel
Clinical Pathology Department, National Cancer Institute, Cairo University, Cairo 11796, Egypt
Hassan Elzohry
Hepatology and Gastroenterology Department, National Liver Institute, Menofia University, Shebin El-Kom 32511, Egypt
Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Interestingly, lncRNA-H19 acts independently in HCC and influences miR-675 expressions. We aimed to assess the potential utility of tissue lncRNA-H19 versus miR-675 expressions as a non-invasive biomarker for HCC diagnosis and prognosis in Egyptian patients. Ninety-one HCC patients and 91 controls included in this study were investigated for expression of lncRNA-H19 and miR675 using RT-qPCR. Our results showed that the expression of lncRNA-H19 and microRNA-675 were higher in patients than in controls (p p p < 0.001, 0.001, respectively). Oncofetal H19-derived miR-675 expression could be considered a potential noninvasive diagnostic and prognostic biomarker, outstanding the performance of the expression of tissue lncRNA-H19 for HCC.
