The Potential Utility of Circulating Oncofetal H19 Derived miR-675 Expression versus Tissue lncRNA-H19 Expression in Diagnosis and Prognosis of HCC in Egyptian Patients

Shimaa Abdelsattar; Dina Sweed; Hala F. M. Kamel; Zeinab A. Kasemy; Abdallah M. Gameel; Hassan Elzohry; Omnia Ameen; Eman Ibrahim Elgizawy; Ahmed Sallam; Asmaa Mosbeh; Mahmoud S. Abdallah; Fatma O. Khalil; Hiba S. Al-Amodi; Sally M. El-Hefnway

doi:10.3390/biom13010003

Biomolecules (Dec 2022)

The Potential Utility of Circulating Oncofetal H19 Derived miR-675 Expression versus Tissue lncRNA-H19 Expression in Diagnosis and Prognosis of HCC in Egyptian Patients

Shimaa Abdelsattar,
Dina Sweed,
Hala F. M. Kamel,
Zeinab A. Kasemy,
Abdallah M. Gameel,
Hassan Elzohry,
Omnia Ameen,
Eman Ibrahim Elgizawy,
Ahmed Sallam,
Asmaa Mosbeh,
Mahmoud S. Abdallah,
Fatma O. Khalil,
Hiba S. Al-Amodi,
Sally M. El-Hefnway

Affiliations

Shimaa Abdelsattar: Clinical Biochemistry and Molecular Diagnostics Department, National Liver Institute, Menofia University, Shebin El-Kom 32511, Egypt
Dina Sweed: Pathology Department, National Liver Institute, Menofia University, Shebin El-Kom 32511, Egypt
Hala F. M. Kamel: Department of Biochemistry, Faculty of Medicine, Umm Al Qura University, Makka 21955, Saudi Arabia
Zeinab A. Kasemy: Public Health and Community Medicine Department, Faculty of Medicine, Menofia University, Shebin El-Kom 32511, Egypt
Abdallah M. Gameel: Clinical Pathology Department, National Cancer Institute, Cairo University, Cairo 11796, Egypt
Hassan Elzohry: Hepatology and Gastroenterology Department, National Liver Institute, Menofia University, Shebin El-Kom 32511, Egypt
Omnia Ameen: Physiology Department, Faculty of Medicine, Menofia University, Shebin El-Kom 32511, Egypt
Eman Ibrahim Elgizawy: Physiology Department, Faculty of Medicine, Menofia University, Shebin El-Kom 32511, Egypt
Ahmed Sallam: Department of Hepatobiliary and Pancreatic Surgery, National Liver Institute, Menofia University, Shebin El-Kom 32511, Egypt
Asmaa Mosbeh: Pathology Department, National Liver Institute, Menofia University, Shebin El-Kom 32511, Egypt
Mahmoud S. Abdallah: Clinical Pharmacy Department, Faculty of Pharmacy, University of Sadat City (USC), Sadat City 32897, Egypt
Fatma O. Khalil: Clinical and Molecular Microbiology and Immunology Department, National Liver Institute, Menoufia University, Shebin El-Kom 32511, Egypt
Hiba S. Al-Amodi: Department of Biochemistry, Faculty of Medicine, Umm Al Qura University, Makka 21955, Saudi Arabia
Sally M. El-Hefnway: Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Menofia University, Shebin El-Kom 32511, Egypt

DOI: https://doi.org/10.3390/biom13010003
Journal volume & issue: Vol. 13, no. 1
p. 3

Abstract

Read online

Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Interestingly, lncRNA-H19 acts independently in HCC and influences miR-675 expressions. We aimed to assess the potential utility of tissue lncRNA-H19 versus miR-675 expressions as a non-invasive biomarker for HCC diagnosis and prognosis in Egyptian patients. Ninety-one HCC patients and 91 controls included in this study were investigated for expression of lncRNA-H19 and miR675 using RT-qPCR. Our results showed that the expression of lncRNA-H19 and microRNA-675 were higher in patients than in controls (p p p < 0.001, 0.001, respectively). Oncofetal H19-derived miR-675 expression could be considered a potential noninvasive diagnostic and prognostic biomarker, outstanding the performance of the expression of tissue lncRNA-H19 for HCC.

Published in Biomolecules

ISSN: 2218-273X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: https://www.mdpi.com/journal/biomolecules

About the journal

Abstract

Keywords