Antimicrobial activity of rosemary-pepper essential oil and barbatimao dry crude extract against bacteria isolated from milk

Abstract

The aim of this study was to evaluate the in vitro antimicrobial activity of Lippia sidoides essential oil (LsEO), popularly known as “rosemary-pepper”, and the Stryphnodendron adstringens dry crude extract (SaDCE), or “barbatimao”, against bacteria isolated from total milk flock, from small farms of northern Minas Gerais state. SaDCE was obtained from the peel of the vegetable through static distillation in ethanol 99.9% during eight days. LsEO was obtained through hydro-distillation of its fresh leaves. The bacteria Escherichia coli and Staphylococcus aureus isolated from milk underwent the test of disk-diffusion and minimum bacterial concentration (MBC), using concentrations of 320, 160, 80, 40, 20 and 0μl/mL of LsEO and 400, 200, 100, 50, 25 and 0mg/mL of SaDCE. All bacteria were sensitive to the vegetable extracts, except the Escherichia coli which was not inhibited by any test when SaDCE was used.

Keywords

• Coliforms
• MBC
• Medicinal plants
• Lippia sidoides
• Stryphnodendron adstringens