Wound Healing Composite Materials of Bacterial Cellulose and Zinc Oxide Nanoparticles with Immobilized Betulin Diphosphate
Nina Melnikova,
Alexander Knyazev,
Viktor Nikolskiy,
Peter Peretyagin,
Kseniia Belyaeva,
Natalia Nazarova,
Elena Liyaskina,
Darina Malygina,
Viktor Revin
Affiliations
Nina Melnikova
Faculty of Chemistry, Lobachevsky University, 23/5 Gagarin Av., 603950 Nizhny Novgorod, Russia
Alexander Knyazev
Faculty of Chemistry, Lobachevsky University, 23/5 Gagarin Av., 603950 Nizhny Novgorod, Russia
Viktor Nikolskiy
Nizhni Novgorod Regional Clinical Hospital named after N.A. Semashko, 190 Rodionova str., 603126 Nizhny Novgorod, Russia
Peter Peretyagin
Department of Experimental Medicine, Privolzhsky Research Medical University, 10/1 Minin sq., 603950 Nizhny Novgorod, Russia
Kseniia Belyaeva
Department of Experimental Medicine, Privolzhsky Research Medical University, 10/1 Minin sq., 603950 Nizhny Novgorod, Russia
Natalia Nazarova
Department of Biotechnology, Bioengineering and Biochemistry, National Research Ogarev Mordovia State University, 68 Bolshevistskaya str., 430005 Saransk, Russia
Elena Liyaskina
Department of Biotechnology, Bioengineering and Biochemistry, National Research Ogarev Mordovia State University, 68 Bolshevistskaya str., 430005 Saransk, Russia
Darina Malygina
Department of Pharmaceutical Chemistry, Privolzhsky Research Medical University, 10/1 Minin sq., 603950 Nizhny Novgorod, Russia
Viktor Revin
Department of Biotechnology, Bioengineering and Biochemistry, National Research Ogarev Mordovia State University, 68 Bolshevistskaya str., 430005 Saransk, Russia
A design of new nanocomposites of bacterial cellulose (BC) and betulin diphosphate (BDP) pre-impregnated into the surface of zinc oxide nanoparticles (ZnO NPs) for the production of wound dressings is proposed. The sizes of crystalline BC and ZnO NPs (5–25%) corresponded to 5–6 nm and 10–18 nm, respectively (powder X-ray diffractometry (PXRD), Fourier-infrared (FTIR), ultraviolet (UV), atomic absorption (AAS) and photoluminescence (PL) spectroscopies). The biological activity of the wound dressings “BC-ZnO NPs-BDP” was investigated in rats using a burn wound model. Morpho-histological studies have shown that more intensive healing was observed during treatment with hydrophilic nanocomposites than the oleophilic standard (ZnO NPs-BDP oleogel; p p < 0.001). The microcirculation index was restored on the 3rd day after burn under treatment with BC-ZnO NPs-BDP wound dressings. The results of effective wound healing with BC-ZnO NPs-BDP nanocomposites can be explained by the synergistic effect of all nanocomposite components, which regulate oxygenation and microcirculation, reducing hypoxia and oxidative stress in a burn wound.
