An in vitro assay to quantify satellite cell activation using isolated mouse myofibers

Rodrigo Canibano-Fraile; Emma Boertjes; Stela Bozhilova; W.W.M. Pim Pijnappel; Gerben J. Schaaf

STAR Protocols (Jun 2021)

An in vitro assay to quantify satellite cell activation using isolated mouse myofibers

Rodrigo Canibano-Fraile,
Emma Boertjes,
Stela Bozhilova,
W.W.M. Pim Pijnappel,
Gerben J. Schaaf

Affiliations

Rodrigo Canibano-Fraile: Department of Clinical Genetics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands; Department of Pediatrics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands; Center for Lysosomal and Metabolic Diseases, Erasmus MC University Medical Center, 3015 GE Rotterdam, the Netherlands
Emma Boertjes: Department of Clinical Genetics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands; Department of Pediatrics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands; Center for Lysosomal and Metabolic Diseases, Erasmus MC University Medical Center, 3015 GE Rotterdam, the Netherlands
Stela Bozhilova: Department of Clinical Genetics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands; Department of Pediatrics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands; Center for Lysosomal and Metabolic Diseases, Erasmus MC University Medical Center, 3015 GE Rotterdam, the Netherlands
W.W.M. Pim Pijnappel: Department of Clinical Genetics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands; Department of Pediatrics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands; Center for Lysosomal and Metabolic Diseases, Erasmus MC University Medical Center, 3015 GE Rotterdam, the Netherlands
Gerben J. Schaaf: Department of Clinical Genetics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands; Department of Pediatrics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands; Center for Lysosomal and Metabolic Diseases, Erasmus MC University Medical Center, 3015 GE Rotterdam, the Netherlands; Corresponding author

Journal volume & issue: Vol. 2, no. 2
p. 100482

Abstract

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Summary: Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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