Generation of four iPSC lines from a family harboring a 1p36–35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling

Gakuya Takamatsu; Yoko Manome; Jun-Seok Lee; Kanako Toyama; Tomoko Hayakawa; Chikako Hara-Miyauchi; Minami Hasegawa-Ogawa; Chiaki Katagiri; Tsuyoshi Kondo; Hirotaka James Okano; Masayuki Matsushita

Stem Cell Research (Oct 2022)

Generation of four iPSC lines from a family harboring a 1p36–35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling

Gakuya Takamatsu,
Yoko Manome,
Jun-Seok Lee,
Kanako Toyama,
Tomoko Hayakawa,
Chikako Hara-Miyauchi,
Minami Hasegawa-Ogawa,
Chiaki Katagiri,
Tsuyoshi Kondo,
Hirotaka James Okano,
Masayuki Matsushita

Affiliations

Gakuya Takamatsu: Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Neuropsychiatry, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan
Yoko Manome: Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan
Jun-Seok Lee: Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan; Advanced Medical Research Center, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan
Kanako Toyama: Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan; Advanced Medical Research Center, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan
Tomoko Hayakawa: Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan; Division of Clinical Pharmacology, Department of Pharmacology, Jichi Medical University, Tochigi, Japan
Chikako Hara-Miyauchi: Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan; Center for Brain Integration Research, Tokyo Medical and Dental University, Tokyo, Japan; Kanagawa Institute of Industrial Science and Technology (KISTEC), Kanagawa, Japan
Minami Hasegawa-Ogawa: Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan
Chiaki Katagiri: Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan; Department of Synbiotics, Institute for Genetic Medicine, Hokkaido University, Hokkaido, Japan
Tsuyoshi Kondo: Department of Neuropsychiatry, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan
Hirotaka James Okano: Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan
Masayuki Matsushita: Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan; Corresponding author.

Journal volume & issue: Vol. 64
p. 102915

Abstract

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Induced pluripotent stem cells (iPSCs) obtained from genetically characterized patients benefit the biological study of bipolar disorder (BD). Here, we present iPSC lines from three-generation patients with BD and recurrent depressive disorder (RDD) and a healthy control sibling in a family. All patients shared the specified haplotype in the 1p36–35, previously reported as the susceptibility locus of mood disorders. iPSCs were generated with the reprogramming factors OTC3/4, l-MYC, LIN28, SOX2, KLF4, and p53 shRNA through non-integrated episomal vectors. All iPSC lines strongly expressed pluripotency markers and proved the ability to differentiate into three germ lineages in vitro.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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