Integrated Analysis of Long Non-Coding RNA Expression Profiles in <i>Glaesserella parasuis</i>-Induced Meningitis: New Insight into Pathogenesis

Peiyan Sun; Yaqiong Yang; Hongxing Cheng; Shulin Fu; Yulan Liu; Yinsheng Qiu; Hongbo Chen; Jing Zhang; Huanhuan Zhou; Liangyu Shi; Hongyan Ren; Zhe Chao; Ling Guo

doi:10.3390/microbiolres14030097

Microbiology Research (Sep 2023)

Integrated Analysis of Long Non-Coding RNA Expression Profiles in <i>Glaesserella parasuis</i>-Induced Meningitis: New Insight into Pathogenesis

Peiyan Sun,
Yaqiong Yang,
Hongxing Cheng,
Shulin Fu,
Yulan Liu,
Yinsheng Qiu,
Hongbo Chen,
Jing Zhang,
Huanhuan Zhou,
Liangyu Shi,
Hongyan Ren,
Zhe Chao,
Ling Guo

Affiliations

Peiyan Sun: Laboratory of Genetic Breeding, School of Animal Science and Nutritional Engineering, Reproduction and Precision Livestock Farming, Wuhan Polytechnic University, Wuhan 430023, China
Yaqiong Yang: Laboratory of Genetic Breeding, School of Animal Science and Nutritional Engineering, Reproduction and Precision Livestock Farming, Wuhan Polytechnic University, Wuhan 430023, China
Hongxing Cheng: Laboratory of Genetic Breeding, School of Animal Science and Nutritional Engineering, Reproduction and Precision Livestock Farming, Wuhan Polytechnic University, Wuhan 430023, China
Shulin Fu: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China
Yulan Liu: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China
Yinsheng Qiu: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China
Hongbo Chen: Laboratory of Genetic Breeding, School of Animal Science and Nutritional Engineering, Reproduction and Precision Livestock Farming, Wuhan Polytechnic University, Wuhan 430023, China
Jing Zhang: Laboratory of Genetic Breeding, School of Animal Science and Nutritional Engineering, Reproduction and Precision Livestock Farming, Wuhan Polytechnic University, Wuhan 430023, China
Huanhuan Zhou: Laboratory of Genetic Breeding, School of Animal Science and Nutritional Engineering, Reproduction and Precision Livestock Farming, Wuhan Polytechnic University, Wuhan 430023, China
Liangyu Shi: Laboratory of Genetic Breeding, School of Animal Science and Nutritional Engineering, Reproduction and Precision Livestock Farming, Wuhan Polytechnic University, Wuhan 430023, China
Hongyan Ren: Key Laboratory of Animal Embryo Engineering and Molecular Breeding of Hubei Province, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China
Zhe Chao: Hainan Key Laboratory for Tropical Animal Breeding and Disease Research, Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou 571100, China
Ling Guo: Laboratory of Genetic Breeding, School of Animal Science and Nutritional Engineering, Reproduction and Precision Livestock Farming, Wuhan Polytechnic University, Wuhan 430023, China

DOI: https://doi.org/10.3390/microbiolres14030097
Journal volume & issue: Vol. 14, no. 3
pp. 1427 – 1441

Abstract

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Glaesserella parasuis (G. parasuis) can elicit meningitis in pigs; however, the pathogenic mechanisms of meningitis induced by G. parasuis remain unclear. Long non-coding RNAs (lncRNAs) have been proven to play key roles in a variety of physiological and pathological processes. However, whether lncRNAs are involved in meningitis triggered by G. parasuis has not been investigated. In this study, we performed an integrative analysis of lncRNAs expression profiles in the porcine brain infected with G. parasuis using RNA-seq. The results showed that lncRNA expressions in G. parasuis-induced meningitis were modified, and a total of 306 lncRNAs exhibited significant differential expression, in which 176 lncRNAs were up-regulated and 130 lncRNAs were down-regulated. KEGG enrichment analysis demonstrated that the differentially expressed target mRNAs of affected lncRNAs in G. parasuis-infected porcine brain were mainly involved in the cell adhesion molecules (CAMs), Jak-STAT signaling pathway, PI3k-Akt signaling pathway, and TNF signaling pathway. The expression relationship between the most affected differential lncRNAs and their differential target mRNAs was visualized by a co-expression network. A protein-protein interaction network consisting of 12 differential targets was constructed using STRING analysis. In addition, differential expressions of important lncRNAs were validated by qRT-PCR. lncRNA ALDBSSCT0000007362, ALDBSSCT0000001959, ALDBSSCT0000005529, MSTRG.2939.1, and MSTRG.32374.1 showed the same expression pattern with the lncRNA sequencing data. Our results demonstrated that G. parasuis could modify the lncRNA expression profiles in the porcine brain. To the best of our knowledge, this is the first report revealing the integrative analysis of lncRNA expression profiles in G. parasuis-induced meningitis, which could enhance important information to understand the inflammatory functions of lncRNAs involved in swine meningitis, and also provide a foundation for finding out novel strategies to prevent and treat meningitis in piglets triggered by G. parasuis.

Published in Microbiology Research

ISSN: 2036-7481 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: https://www.mdpi.com/journal/microbiolres/

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