Nature Communications (Nov 2021)

Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii

  • Aron Ferenczi,
  • Yen Peng Chew,
  • Erika Kroll,
  • Charlotte von Koppenfels,
  • Andrew Hudson,
  • Attila Molnar

DOI
https://doi.org/10.1038/s41467-021-27004-1
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 12

Abstract

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Single-stranded oligodeoxynucleotides are often used as templates for DNA repair during genome editing. Here the authors show that, unlike in animals, single-strand templated DNA repair in Chlamydomonas reinhardtii relies on the alternative end-joining enzyme polymerase θ.