PROGRESSION OF ‘OMICS’ METHODOLOGIES FOR UNDERSTANDING THE PATHOGENICITY OF CORYNEBACTERIUM PSEUDOTUBERCULOSIS: THE BRAZILIAN EXPERIENCE

Abstract

Since the first successful attempt at sequencing the Corynebacterium pseudotuberculosis genome, large amounts of genomic, transcriptomic and proteomic data have been generated. C. pseudotuberculosis is an interesting bacterium due to its great zoonotic potential and because it causes considerable economic losses worldwide. Furthermore, different strains of C. pseudotuberculosis are capable of causing various diseases in different hosts. Currently, we seek information about the phylogenetic relationships between different strains of C. pseudotuberculosis isolates from different hosts across the world and to employ these data to develop tools to diagnose and eradicate the diseases these strains cause. In this review, we present the latest findings on C. pseudotuberculosis that have been obtained with the most advanced techniques for sequencing and genomic organization. We also discuss the development of in silico tools for processing these data to prompt a better understanding of this pathogen.

Keywords

• Corynebacterium pseudotuberculosis
• SOLiD next generation sequencing
• Ion Torrent next generation sequencing
• SDS-PAGE
• mass spectrometry
• RNA-seq