HIV-1 interaction with an O-glycan-specific bacterial lectin enhances virus infectivity and resistance to neutralizing antibodies
Daniel W. Heindel,
Dania M. Figueroa Acosta,
Marisa Goff,
Clauvis Kunkeng Yengo,
Muzafar Jan,
Xiaomei Liu,
Xiao-Hong Wang,
Mariya I. Petrova,
Mo Zhang,
Manish Sagar,
Phillip Barnette,
Shilpi Pandey,
Ann J. Hessell,
Kun-Wei Chan,
Xiang-Peng Kong,
Benjamin K. Chen,
Lara K. Mahal,
Barbara A. Bensing,
Catarina E. Hioe
Affiliations
Daniel W. Heindel
Divison of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA
Dania M. Figueroa Acosta
Divison of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA
Marisa Goff
Divison of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA
Clauvis Kunkeng Yengo
Divison of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA
Muzafar Jan
Divison of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA
Xiaomei Liu
Divison of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA
Xiao-Hong Wang
VA New York Harbor Healthcare System-Manhattan, New York, NY, USA
Mariya I. Petrova
Department of Bioscience Engineering, University of Antwerp, Antwerp, Belgium
Mo Zhang
Department of Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA
Manish Sagar
Department of Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA; Department of Virology, Immunology and Microbiology, Boston University Chobanian & Avedisian School of Medicine, Boston, MA, USA
Phillip Barnette
Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA
Shilpi Pandey
Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA
Ann J. Hessell
Division of Pathobiology and Immunology, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA
Kun-Wei Chan
Department of Biochemistry and Molecular Pharmacology New York University Grossman School of Medicine, New York, NY, USA
Xiang-Peng Kong
Department of Biochemistry and Molecular Pharmacology New York University Grossman School of Medicine, New York, NY, USA
Benjamin K. Chen
Divison of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA
Lara K. Mahal
Department of Chemistry, University of Alberta, Edmonton, AB, Canada
Barbara A. Bensing
Department of Medicine, San Francisco Veterans Affairs Medical Center and University of California, San Francisco, CA, USA
Catarina E. Hioe
Divison of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA; James J. Peters VA Medical Center, Bronx, NY, USA; Corresponding author
Summary: Bacteria dysbiosis and its accompanying inflammation or compromised mucosal integrity is associated with an increased risk of HIV-1 transmission. However, HIV-1 may also bind bacteria or bacterial products to impact infectivity and transmissibility. This study evaluated HIV-1 interactions with bacteria through glycan-binding lectins. The Streptococcal Siglec-like lectin SLBR-N, a part of the fimbriae shrouding the bacteria surface that recognizes α2,3 sialyated O-linked glycans, was noted for its ability to enhance HIV-1 infectivity in the context of cell-free infection and cell-to-cell transfer. Enhancing effects were recapitulated with O-glycan-binding plant lectins, signifying the importance of O-glycans. N-glycan-binding bacterial lectins FimH and Msl had no effect. SLBR-N was demonstrated to capture and transfer infectious HIV-1 virions, bind to O-glycans on HIV-1 Env, and increase HIV-1 resistance to neutralizing antibodies targeting different regions of Env. This study highlights the potential contribution of O-glycan-binding lectins from commensal bacteria at the mucosa in promoting HIV-1 infection.
