Acta Cirúrgica Brasileira (Mar 2017)

Effects of hypertonic saline solution associated to remote ischemic perconditioning in kidney ischemia/reperfusion injury in rats

  • Marcus Vinicius Henriques Brito,
  • Edson Yuzur Yasojima,
  • Sandro Percário,
  • Rubens Fernando Gonçalves Ribeiro Júnior,
  • Lainy Carollyne da Costa Cavalcante,
  • Andrew Moraes Monteiro,
  • Rodrigo Paracampo Couteiro,
  • Ivone Aline da Silva Rodrigues,
  • Hellen Aparecida Geyer dos Santos

DOI
https://doi.org/10.1590/s0102-865020170030000005
Journal volume & issue
Vol. 32, no. 3
pp. 211 – 218

Abstract

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Abstract Purpose: To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in renal ischemia/reperfusion injury in rats. Methods: Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. After reperfusion, blood samples were collected for BUN and creatinine serum levels analyzes. TBARS were evaluated in plasma and renal tissue to assess oxidative stress. Kidney histopathological examination were performed. Results: Per+HSS group showed a lower degree of renal dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly reduced oxidative stress and histological damage. Conclusion: Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute renal injury induced by ischemia/reperfusion.

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