Frontiers in Endocrinology (Feb 2022)

Biomarker Value of miR-221 and miR-222 as Potential Substrates in the Differential Diagnosis of Papillary Thyroid Cancer Based on Data Synthesis and Bioinformatics Approach

  • Shang Cai,
  • Shang Cai,
  • Jiayan Ma,
  • Yong Wang,
  • Yong Wang,
  • Yuxing Cai,
  • Yuxing Cai,
  • Liwei Xie,
  • Liwei Xie,
  • Xiangying Chen,
  • Xiangying Chen,
  • Yingying Yang,
  • Yingying Yang,
  • Qiliang Peng,
  • Qiliang Peng

DOI
https://doi.org/10.3389/fendo.2021.794490
Journal volume & issue
Vol. 12

Abstract

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BackgroundMicroRNA (miRNA) has been reported to play a critical regulatory role in papillary thyroid carcinomas (PTC). However, the role of miR-221/222 in PTC remains unclear. Here, we performed this study to explore the diagnostic potentials and mechanisms of miR-221/222 in PTC.MethodsFirst, we systematically analyzed the diagnostic value of miR-221/222 in the diagnosis PTC by pooling the published studies. Afterwards, we performed comprehensive bioinformatics analysis including gene ontology analysis, pathway enrichment analysis and protein-protein interaction analysis to explore the potential mechanisms of miR-221/222 involved in PTC.ResultsThe overall sensitivity and specificity of miR-221/222 for PTC were 0.75 (95% CI: 0.70–0.80) and 0.80 (95% CI: 0.76–0.84) respectively with the AUC of 0.85 (95% CI: 0.81-0.88). The diagnostic performance varied among different subgroups including geographical locations, sample sources and sample sizes. Meanwhile, we found that a combination of miR-221/222 and other miRNAs when used in a diagnostic panel could improve the diagnostic accuracy than individual miR-221/222. Moreover, through the bioinformatics analysis, we confirmed that miR-221/222 targets were highly related to the molecular pathogenesis of PTC. The results revealed that miR-221/222 may exert important functions in PTC through thyroid hormone signaling pathway and some other key pathways by regulating some key genes.ConclusionThese findings indicated that miR-221/222 have the potential to serve as auxiliary tools for diagnosing PTC. Further prospective clinical trials should be performed to assess the accuracy of these findings in a larger cohort and determine the clinical uses.

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