Analysis of cytotoxicity of laser ablated nanoparticles on oral cancer cell (OECM-1) - An In vitro study

Abstract

Background: Drug delivery systems employing nanoparticles (NPs) have demonstrated numerous benefits in the treatment of cancer. The past decade has witnessed extensive discussions regarding the prominent therapeutic and diagnostic properties of inorganic NPs in the field of oncology. Objectives: This study aims at formulating copper, gold, and silver NPs by the laser ablation technique and assessing the impact of NPs on cancerous cells. Methodology: Characterization of NPs was done by energy-dispersive X-ray analysis, and their particle size distribution was studied by dynamic light scattering. The confirmation of copper NPs was done by using a UV–visible spectrometer. The biological effect of NPs on cancer cells was studied by sulforhodamine B (SRB) assay. Results: In this study, it was found that there was 43% effectiveness of NP treatment to subjugate cancerous cells, whereas the normal toxic procedure is 78% effective after 48 hours treatment with side effects, which will hamper day-to-day activity of the patient. Conclusion: Side effects with our treatment protocol are rapidly mitigated as normal cells are not affected. This study presents promising outcomes and suggests that at greater concentrations, NPs may enhance efficacy, warranting additional research in this arena.

Keywords

• ablation techniques
• cancer
• cytotoxic
• laser
• nanoparticles