Patologìâ (Aug 2016)
Effect of Pro12Ala polymorphism of PPARG gene on lipid peroxidation and antioxidant defense in patients with type 2 diabetes depending on the duration of the disease
Abstract
Aim – to study the impact of polymorphic marker rs1801282 of gene PPARG on the formation of oxidative stress in terms of LPO and AOS in patients with type 2 diabetes depending on the duration of the disease. Materials and methods. The study was conducted involving 138 patients, 88 of whom were patients with type 2 diabetes and the control group consisted of 50 people. Patients were divided into three groups according to disease duration: 5 years, 5-10 years and more than 10 years. LPO activity was assessed in terms of diene conjugates (DC) and malonic dialdehyde (MDA) and AOC status - the activity of superoxide dismutase (SOD), catalase and the level of α-tocopherol (α-TF). Molecular genetic studies were conducted by the method of PCR in real time, and to analyze polymorphic DNA loci a standardized test system TaqMan Mutation Detection Assays Life-Technology (USA) was used. Results. During 5-10 years disease levels of DC and MDA in patients with Pro12Ala genotype were increased by 34,9% and 34,7%, as compared to Pro12Pro (p=0,01). Availability of Pro12Pro stipulated the reduction of catalase activity during 5-10 years disease by 75% (p=0,001), and for those, who are ill for more than 10 years by 2,04 times (p=0,01), which is not different from the reference level (F=1,19; p=0,600), but in the case of Pro12Ala, this figure was 2 times higher. Conclusions. Pro12Ala polymorphism (rs1801282) of PPARG gene causes the development of oxidative stress in type 2 diabetes with 5-10 years durations, and genotype Pro12Pro - lack of the catalase enzyme level of antioxidant system in patients with durations of disease more than 5 years.
Keywords
