Application of novel label-free amperometric biosensing platform for determination of lactate in saliva samples during sport practice

Abstract

Herein, novel amperometric based biosensor using a MXene-Pt nanocomposite was fabricated and utilized for lactate analysis. MXene-Pt provided a desirable matrix for the anchoring of lactate oxidase (LOD). Excellent electron transfer occurred at the interface between the receptor and glassy carbon electrode (GCE) due to the synergetic effect between MXene layers and Pt nanoparticles (NPs). The uniform distribution of small sized Pt NPs gives rise to superior electron transfer kinetics, thereby maximizing the lactate detection abilities of the LOD-MXene-Pt modified GCE. The novel sensing platform displays this linear response in buffer medium (pH = 7.4) within the range of concentrations of 0.02–0.75 mM, and the detection limit of 10 µM based on 3σ was obtained for lactate detection. The accurate analysis of lactate in physiological samples indicated that the presence method is a very specific tool. It was used for successful measurement of lactate concentration in saliva samples during sport practice. The proposed strategy is convenient and less expensive, quick analysis time and offers an efficient procedure of lactate monitoring in the common clinical tests.

Keywords

• Biosensor fabrication
• 2D MXene
• Lactate detection
• Pt nanoparticles
• Sport