Scientific Reports (Jun 2021)
Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand
- Nonlawat Boonyalai,
- Chatchadaporn Thamnurak,
- Piyaporn Sai-ngam,
- Winita Ta-aksorn,
- Montri Arsanok,
- Nichapat Uthaimongkol,
- Siratchana Sundrakes,
- Sorayut Chattrakarn,
- Chaiyaporn Chaisatit,
- Chantida Praditpol,
- Watcharintorn Fagnark,
- Kirakarn Kirativanich,
- Suwanna Chaorattanakawee,
- Pattaraporn Vanachayangkul,
- Paphavee Lertsethtakarn,
- Panita Gosi,
- Darunee Utainnam,
- Wuttikon Rodkvamtook,
- Worachet Kuntawunginn,
- Brian A. Vesely,
- Michele D. Spring,
- Mark M. Fukuda,
- Charlotte Lanteri,
- Douglas Walsh,
- David L. Saunders,
- Philip L. Smith,
- Mariusz Wojnarski,
- Narongrid Sirisopana,
- Norman C. Waters,
- Krisada Jongsakul,
- Jariyanart Gaywee
Affiliations
- Nonlawat Boonyalai
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Chatchadaporn Thamnurak
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Piyaporn Sai-ngam
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Winita Ta-aksorn
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Montri Arsanok
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Nichapat Uthaimongkol
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Siratchana Sundrakes
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Sorayut Chattrakarn
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Chaiyaporn Chaisatit
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Chantida Praditpol
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Watcharintorn Fagnark
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Kirakarn Kirativanich
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Suwanna Chaorattanakawee
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Pattaraporn Vanachayangkul
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Paphavee Lertsethtakarn
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Panita Gosi
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Darunee Utainnam
- Royal Thai Army Component, Armed Forces Research Institute of Medical Sciences
- Wuttikon Rodkvamtook
- Royal Thai Army Component, Armed Forces Research Institute of Medical Sciences
- Worachet Kuntawunginn
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Brian A. Vesely
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Michele D. Spring
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Mark M. Fukuda
- Department of Retrovirology, Armed Forces Research Institute of Medical Sciences
- Charlotte Lanteri
- Walter Reed Army Institute of Research
- Douglas Walsh
- Department of Dermatology, Syracuse VA medical center
- David L. Saunders
- U.S. Army Research Institute of Infectious Diseases
- Philip L. Smith
- Walter Reed Army Institute of Research
- Mariusz Wojnarski
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Narongrid Sirisopana
- Royal Thai Army Component, Armed Forces Research Institute of Medical Sciences
- Norman C. Waters
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Krisada Jongsakul
- Department of Bacterial and Parasitic Diseases, Armed Forces Research Institute of Medical Sciences
- Jariyanart Gaywee
- Royal Thai Army Component, Armed Forces Research Institute of Medical Sciences
- DOI
- https://doi.org/10.1038/s41598-021-92735-6
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 13
Abstract
Abstract Malaria remains a public health problem in Thailand, especially along its borders where highly mobile populations can contribute to persistent transmission. This study aimed to determine resistant genotypes and phenotypes of 112 Plasmodium falciparum isolates from patients along the Thai-Cambodia border during 2013–2015. The majority of parasites harbored a pfmdr1-Y184F mutation. A single pfmdr1 copy number had CVIET haplotype of amino acids 72–76 of pfcrt and no pfcytb mutations. All isolates had a single pfk13 point mutation (R539T, R539I, or C580Y), and increased % survival in the ring-stage survival assay (except for R539I). Multiple copies of pfpm2 and pfcrt-F145I were detected in 2014 (12.8%) and increased to 30.4% in 2015. Parasites containing either multiple pfpm2 copies with and without pfcrt-F145I or a single pfpm2 copy with pfcrt-F145I exhibited elevated IC90 values of piperaquine. Collectively, the emergence of these resistance patterns in Thailand near Cambodia border mirrored the reports of dihydroartemisinin-piperaquine treatment failures in the adjacent province of Cambodia, Oddar Meanchey, suggesting a migration of parasites across the border. As malaria elimination efforts ramp up in Southeast Asia, host nations militaries and other groups in border regions need to coordinate the proposed interventions.
