Amelioration of human acute lymphoblastic leukemia (ALL) cells by ZnO-TiO2-Chitosan-Amygdalin nanocomposites

Abozer Y. Elderdery; Badr Alzahrani; Fehaid Alanazi; Siddiqa M.A. Hamza; Ahmed M.E. Elkhalifa; Abdulaziz H. Alhamidi; Abdulrahim A. Alabdulsalam; A. Mohamedain; Suresh S. Kumar; Pooi Ling Mok

Arabian Journal of Chemistry (Aug 2022)

Amelioration of human acute lymphoblastic leukemia (ALL) cells by ZnO-TiO2-Chitosan-Amygdalin nanocomposites

Abozer Y. Elderdery,
Badr Alzahrani,
Fehaid Alanazi,
Siddiqa M.A. Hamza,
Ahmed M.E. Elkhalifa,
Abdulaziz H. Alhamidi,
Abdulrahim A. Alabdulsalam,
A. Mohamedain,
Suresh S. Kumar,
Pooi Ling Mok

Affiliations

Abozer Y. Elderdery: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia; Health Sciences Research Unit, Jouf University, Sakaka, Saudi Arabia; Corresponding author at: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia.
Badr Alzahrani: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia
Fehaid Alanazi: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences-AlQurayyat, Jouf University, Saudi Arabia
Siddiqa M.A. Hamza: Department of Pathology, Umm Alqura University, Algunfuda, Saudi Arabia
Ahmed M.E. Elkhalifa: Department of Public Health, College of Health Sciences, Saudi Electronic University, Riyadh, Saudi Arabia; Department of Haematology, Faculty of Medical Laboratory Sciences, University of El Imam El Mahdi, Kosti, Sudan
Abdulaziz H. Alhamidi: Clinical Laboratory Sciences Department, College of Applied Medical Science, King Saud University, Riyadh, Saudi Arabia
Abdulrahim A. Alabdulsalam: Department of Biomedical Sciences, College of Medicine, King Faisal University, Alhofuf, Saudi Arabia
A. Mohamedain: Department of Biomedical Sciences, College of Medicine, King Faisal University, Alhofuf, Saudi Arabia; Department of Biochemistry, Faculty of Medicine, Khartoum University, Sudan
Suresh S. Kumar: Centre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research, Chennai, India
Pooi Ling Mok: Department of Biomedical Science, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Journal volume & issue: Vol. 15, no. 8
p. 103999

Abstract

Read online

The present study aims to study the cytotoxicity of ZnO-TiO2-Chitosan-Amygdalin nanocomposites (ZnO-TiO2-Chitosan-Amygdalin) on T lymphoblast cancer cells (MOLT-4). In a study, nanocomposites containing 2.5 to 15 µg/ml MTT were screened for their anticancer activity. Its anticancer properties were significantly higher than those of other nanocomposites with an IC50 value of 10.34 µg/ml. We studied the mechanism of action for cytotoxic cell death by fluorescence microscopy using Acridine Orange/EtBr (AO/EtBr) and Rhodamine 123 staining procedures. Using DCFH-DA, ZnO-TiO2-Chitosan-Amygdalin nanocomposites were analyzed to determine ROS production. The change in apoptotic protein expression for the 24 h following treatment with MOLT-4 cells for Caspase-3, 8, and 9. Nanocomposites containing ZnO-TiO2-Chitosan-Amygdalin increased the number of early and late apoptotic cells in MOLT-4 cells. ZnO-TiO2-Chitosan-Amygdalin nanocomposites also enhanced mitochondrial apoptosis through Caspase cascade signaling. MOLT-4 cells phosphorylated Caspase cascade in response to ZnO-TiO2-Chitosan-Amygdalin nanocomposites. Compared to the control group, the cancer cells treated with ZnO-TiO2-Chitosan-Amygdalin nanocomposites significantly arrest the proliferation and induces cleavage of pro-apoptotic proteins which leads to apoptotic cell death. Accordingly, ZnO-TiO2-Chitosan-Amygdalin nanocomposites might be effective against T lymphoblast cancer.

Published in Arabian Journal of Chemistry

ISSN: 1878-5352 (Print)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry
Website: http://www.journals.elsevier.com/arabian-journal-of-chemistry/

About the journal

Abstract

Keywords