Correlations between the Phylogenetic Relationship of 14 Tulasnella Strains and Their Promotion Effect on Dendrobium crepidatum Protocorm

Jiayi Zhao; Zhenjian Li; Siyu Wang; Fu Yang; Lubin Li; Lei Liu

doi:10.3390/horticulturae8121213

Horticulturae (Dec 2022)

Correlations between the Phylogenetic Relationship of 14 Tulasnella Strains and Their Promotion Effect on Dendrobium crepidatum Protocorm

Jiayi Zhao,
Zhenjian Li,
Siyu Wang,
Fu Yang,
Lubin Li,
Lei Liu

Affiliations

Jiayi Zhao: Key Laboratory of Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Zhenjian Li: Key Laboratory of Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Siyu Wang: Key Laboratory of Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Fu Yang: Key Laboratory of Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Lubin Li: Key Laboratory of Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
Lei Liu: Key Laboratory of Silviculture of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

DOI: https://doi.org/10.3390/horticulturae8121213
Journal volume & issue: Vol. 8, no. 12
p. 1213

Abstract

Read online

The compatibility of mycorrhizal fungi with the early growth stage of orchids is essential for their growth. In this study, the compatibility and promotion effects of 14 Tulasnella strains from different hosts were studied by co-culturing them with the protocorms of Dendrobium crepidatum, which has high ornamental and economic value in China. The ITS–LSU–SSU–TEF combined sequence analysis divided the 14 strains into three clades belonging to Tulasnella calospora (clades A and B) and Tulasnella asymmetrica (clade C). All the strains were compatible with D. crepidatum protocorms within 90 d of the co-culture. Strain T12 in Clade A had a significantly higher (p D. crepidatum, and strain T13 in Clade C had a significantly lower (p D. crepidatum. The findings of this study will contribute to the identification of Tulasnella strains, conservation of D. crepidatum resources, and commercial utilization of mycorrhizal technology.

Published in Horticulturae

ISSN: 2311-7524 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: http://www.mdpi.com/journal/horticulturae

About the journal

Abstract

Keywords