Al Ameen Journal of Medical Sciences (May 2009)
Effect of Tumor Necrosis Factor (TNF) On Survivin Expression In NCI-H520 Cell Strain
Abstract
To investigate inhibitory effect of tumor necrosis factor (TNF) on survivin expression in NCI-H520 cell strain. Methods: The NCI-H520 cell strains were treated with TNF at different concentrations. Assayed inhibitory ratio by MTT), mRNA expression by semiquantitative RT-PCR, and expression of survivin by Western-blotting. Results: TNF obviously inhibited the growth of NCI-H520 cell strain, depending on drug concentration. Survivin expression was degraded after treated with TNF for 4 hours, while the expression of survivin mRNA had no obviously changes in NCI-H520 cell strain. Conclusion: TNF can inhibit the growth of NCI-H520 cell strain, which is concentration-dependent and time-dependent. Besides, TNF can decline the expression of Survivin protein, while the expressions of survivin mRNA have no obviously variation.
