Survey of Pure Rose Water Extract Effect on Male Mice Reproduction

Abstract

Background: Rosa damascene constitutes showed different properties like antimicrobial, anti asthma and bronchitis, anti depression, and skin protection. Objective: Survey of effect of rose water extract on male mice reproductive parameter. Methods: In first part of experiment, adult male mice (Balb/c) 25 - 30 gram were divided into three groups; control, 10 and 20 ml/kg/day rose water. Interaperitoneal injections were done for 7 days and from fifth day, male mice were housed with females. Pregnant female were recognized every morning. In second part of experiment, mice were killed and cauda epididymis were dissected and minced in DMEM/F12 medium. One of rose water doses (5, 10, 50, 100 µlit/ml) added to sperm sample and their motion were assessed. Data were analyzed by one way ANOVA and p<0.05 were considered significant. Results: There is no significant difference in fertilization rate between control and case groups in first experiment, mating rate decreased in cases groups. In second experiment, rose water decreased progressive motion of sperm significantly and changed sperm morphology. Conclusion: Rose water exerts negative effect on sperm motion and morphology and decrease fertilization rate.

Keywords

• rose water
• sperm
• fertilization
• male mice