Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging

Martin L. Tomov; Alison O’Neil; Hamdah S. Abbasi; Beth A. Cimini; Anne E. Carpenter; Lee L. Rubin; Mark Bathe

doi:10.1038/s42003-021-02276-x

Communications Biology (Jun 2021)

Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging

Martin L. Tomov,
Alison O’Neil,
Hamdah S. Abbasi,
Beth A. Cimini,
Anne E. Carpenter,
Lee L. Rubin,
Mark Bathe

Affiliations

Martin L. Tomov: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard
Alison O’Neil: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard
Hamdah S. Abbasi: Imaging Platform at Broad Institute of MIT and Harvard
Beth A. Cimini: Imaging Platform at Broad Institute of MIT and Harvard
Anne E. Carpenter: Imaging Platform at Broad Institute of MIT and Harvard
Lee L. Rubin: Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard
Mark Bathe: Department of Biological Engineering, MIT

DOI: https://doi.org/10.1038/s42003-021-02276-x
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 9

Abstract

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Tomov et al. utilize DNA-PRISM to allow for multiplexed imaging of cultured cells using antibodies modified with oligonucleotide probes. The differentiation of iPSCs to cortical and motor neurons is characterized in model cultures, relevant for use in disease research and drug screening.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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