Ca2+-mediated higher-order assembly of heterodimers in amino acid transport system b0,+ biogenesis and cystinuria

Yongchan Lee; Pattama Wiriyasermkul; Pornparn Kongpracha; Satomi Moriyama; Deryck J. Mills; Werner Kühlbrandt; Shushi Nagamori

doi:10.1038/s41467-022-30293-9

Nature Communications (May 2022)

Ca2+-mediated higher-order assembly of heterodimers in amino acid transport system b0,+ biogenesis and cystinuria

Yongchan Lee,
Pattama Wiriyasermkul,
Pornparn Kongpracha,
Satomi Moriyama,
Deryck J. Mills,
Werner Kühlbrandt,
Shushi Nagamori

Affiliations

Yongchan Lee: Department of Structural Biology, Max Planck Institute of Biophysics
Pattama Wiriyasermkul: Department of Laboratory Medicine, The Jikei University School of Medicine
Pornparn Kongpracha: Department of Laboratory Medicine, The Jikei University School of Medicine
Satomi Moriyama: Department of Collaborative Research for Bio-Molecular Dynamics, Nara Medical University
Deryck J. Mills: Department of Structural Biology, Max Planck Institute of Biophysics
Werner Kühlbrandt: Department of Structural Biology, Max Planck Institute of Biophysics
Shushi Nagamori: Department of Laboratory Medicine, The Jikei University School of Medicine

DOI: https://doi.org/10.1038/s41467-022-30293-9
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 19

Abstract

Read online

Cystinuria is caused by mutations in heterodimeric amino acid transporter known as system b0,+. Here, authors discover that Ca2+ stabilizes the interface between two system b0,+ regulatory subunits rBAT, leading to super-dimerization of the b0,+AT–rBAT heterodimer, facilitating system b0,+ maturation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal