Protocol to monitor cardiac function and hemodynamics in septic rodents

Binbin Meng; Xinrun Wang; Li Li; Haisong Zhang; Wenchao Li; Zhonghua Hu; Lina Zhang; Zhaoxin Qian

STAR Protocols (Mar 2024)

Protocol to monitor cardiac function and hemodynamics in septic rodents

Binbin Meng,
Xinrun Wang,
Li Li,
Haisong Zhang,
Wenchao Li,
Zhonghua Hu,
Lina Zhang,
Zhaoxin Qian

Affiliations

Binbin Meng: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China
Xinrun Wang: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China
Li Li: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China
Haisong Zhang: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China
Wenchao Li: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China
Zhonghua Hu: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Institute of Molecular Precision Medicine and Hunan Key Laboratory of Molecular Precision Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Key Laboratory of Animal Models for Human Diseases, School of Life Sciences, Central South University, Changsha, Hunan 410008, China; Corresponding author
Lina Zhang: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Corresponding author
Zhaoxin Qian: Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Corresponding author

Journal volume & issue: Vol. 5, no. 1
p. 102942

Abstract

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Summary: Septic cardiomyopathy is associated with high mortality in septic patients, characterized by reversible systolic and diastolic dysfunction. It is essential to monitor cardiac function and hemodynamic changes in septic animals. Here, we present a protocol to monitor cardiac function and hemodynamics in septic rodents. We describe steps for performing cecal ligation and puncture on rodents to induce sepsis, acquiring two-dimensional echocardiographic and M-mode ultrasonic images, and assessing mean arterial pressure in septic animals.For complete details on the use and execution of this protocol, please refer to Zhang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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