Scientific Reports (May 2017)

The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides

  • Filip Bartnicki,
  • Piotr Bonarek,
  • Ewa Kowalska,
  • Wojciech Strzalka

DOI
https://doi.org/10.1038/s41598-017-02432-6
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 12

Abstract

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Abstract The discovery of cell penetrating peptides (CPPs) opened new perspectives for the delivery of proteins into human cells. It is considered that in the future CPP-mediated transport of therapeutic proteins may find applications in the treatment of human diseases. Despite this fact a fast and simple method for the purification of CPP-tagged proteins, free of additional tags, was not available to date. To fill this gap we developed the Argi system for one-step purification of proteins tagged with arginine rich CPPs.