Nature Communications (Jul 2020)

Real-time 3D single molecule tracking

  • Shangguo Hou,
  • Jack Exell,
  • Kevin Welsher

DOI
https://doi.org/10.1038/s41467-020-17444-6
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 10

Abstract

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Single molecule observation has been limited to tethered molecules to ensure that the target remains in the field of view (FOV). Here, the authors develop a real-time tracking method that locks onto rapidly diffusing targets and tracks them in a 3D volume, enabling single molecules to remain in the FOV for minutes at a time.