Genetic Mapping and QTL Analysis of Stigma Color in Melon (Cucumis melo L.)

Yuanzuo Lv; Yuanzuo Lv; Peng Gao; Peng Gao; Shi Liu; Shi Liu; Xufeng Fang; Xufeng Fang; Taifeng Zhang; Taifeng Zhang; Tai Liu; Tai Liu; Sikandar Amanullah; Sikandar Amanullah; Xinying Wang; Xinying Wang; Feishi Luan; Feishi Luan

doi:10.3389/fpls.2022.865082

Frontiers in Plant Science (May 2022)

Genetic Mapping and QTL Analysis of Stigma Color in Melon (Cucumis melo L.)

Yuanzuo Lv,
Yuanzuo Lv,
Peng Gao,
Peng Gao,
Shi Liu,
Shi Liu,
Xufeng Fang,
Xufeng Fang,
Taifeng Zhang,
Taifeng Zhang,
Tai Liu,
Tai Liu,
Sikandar Amanullah,
Sikandar Amanullah,
Xinying Wang,
Xinying Wang,
Feishi Luan,
Feishi Luan

Affiliations

Yuanzuo Lv: Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin, China
Yuanzuo Lv: College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China
Peng Gao: Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin, China
Peng Gao: College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China
Shi Liu: Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin, China
Shi Liu: College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China
Xufeng Fang: Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin, China
Xufeng Fang: College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China
Taifeng Zhang: Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin, China
Taifeng Zhang: College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China
Tai Liu: Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin, China
Tai Liu: College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China
Sikandar Amanullah: Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin, China
Sikandar Amanullah: College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China
Xinying Wang: Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin, China
Xinying Wang: College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China
Feishi Luan: Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin, China
Feishi Luan: College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China

DOI: https://doi.org/10.3389/fpls.2022.865082
Journal volume & issue: Vol. 13

Abstract

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Melon is an important Cucurbitaceae crop. Field observations had shown that the green stigmas of melon are more attractive to pollinators than yellow stigmas. In this study, F2 and F2:3 populations obtained by crossing MR-1 (green stigma) and M4-7 (yellow stigma) were used for genetic analysis and mapping. A genetic map of 1,802.49 cm was constructed with 116 cleaved amplified polymorphism sequence (CAPS) markers. Two stable quantitative trait loci (QTLs) linked to the trait of stigma color were identified on chromosomes 2 (SC2.1) and 8 (SC8.1), respectively. An expanded F2 population was used to narrow down the confidence regions of SC2.1 and SC8.1. As a result, SC2.1 was further mapped to a 3.6 cm region between CAPS markers S2M3 and S2B1-3, explaining 9.40% phenotypic variation. SC8.1 was mapped to a 3.7-cm region between CAPS markers S8E7 and S8H-1, explaining 25.92% phenotypic variation. This study broadens our understanding of the mechanisms of stigma color regulation and will be of benefit to the breeding of melon.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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