LINC01089 Blocks the Proliferation and Metastasis of Colorectal Cancer Cells via Regulating miR-27b-3p/HOXA10 Axis

Abstract

Ming Li,1 Xufeng Guo2 1Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430000, Hubei, People’s Republic of China; 2Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430000, Hubei, People’s Republic of ChinaCorrespondence: Xufeng GuoCancer Center, Renmin Hospital of Wuhan University, Wuhan 430000, Hubei, People’s Republic of ChinaEmail [email protected]: An increasing number of studies demonstrate that long non-coding RNAs (lncRNAs) are regulators in cancer biology. Nevertheless, the expression and mechanism of LINC01089 in colorectal cancer (CRC) remain unclear.Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was taken to investigate the expression levels of LINC01089 and miR-27b-3p in CRC tissues and cells. MTT method and transwell test were employed to assess the proliferation and invasion of CRC cells, respectively. Dual-luciferase activity reporter assay, RNA immunoprecipitation assay, Pearson’s correlation analysis, and Western blot were performed to investigate the regulatory mechanism of LINC01089/miR-27b-3p/HOXA10 axis in CRC.Results: LINC01089 was down-regulated in CRC tissues and cell lines. LINC01089 overexpression impeded the proliferation and invasion of SW620 and LoVo cells, whereas LINC01089 knockdown increased the malignancy of SW480 and HT29 cells. Moreover, LINC01089 directly interacted with miR-27b-3p to repressed its expression and indirectly promoted the expression of HOXA10.Conclusion: LINC01089 impedes the proliferation and invasion of colorectal cancer cells by adsorbing miR-27b-3p and up-regulating the expression of HOXA10.Keywords: LINC01089, CRC, miR-27b-3p, HOXA10

Keywords

• linc01089
• crc
• mir-27b-3p
• hoxa10