Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of <i>Saussurea controversa</i>
Igor Khlusov,
Elena Avdeeva,
Valeria Shupletsova,
Olga Khaziakhmatova,
Larisa Litvinova,
Ekaterina Porokhova,
Yaroslav Reshetov,
Irina Zvereva,
Lyudmila Mushtovatova,
Mariia Karpova,
Artem Guryev,
Irina Sukhodolo,
Mikhail Belousov
Affiliations
Igor Khlusov
Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk 634050, Russia
Elena Avdeeva
Department of Pharmaceutical Analysis, Siberian State Medical University, Tomsk 634050, Russia
Valeria Shupletsova
Basic Laboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, Russia
Olga Khaziakhmatova
Basic Laboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, Russia
Larisa Litvinova
Basic Laboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236041, Russia
Ekaterina Porokhova
Department of Morphology and General Pathology, Siberian State Medical University, Tomsk 634050, Russia
Yaroslav Reshetov
Department of Pharmaceutical Analysis, Siberian State Medical University, Tomsk 634050, Russia
Irina Zvereva
Department of Microbiology and Virology, Siberian State Medical University, Tomsk 634050, Russia
Lyudmila Mushtovatova
Department of Microbiology and Virology, Siberian State Medical University, Tomsk 634050, Russia
Mariia Karpova
Department of Microbiology and Virology, Siberian State Medical University, Tomsk 634050, Russia
Artem Guryev
Department of Pharmaceutical Analysis, Siberian State Medical University, Tomsk 634050, Russia
Irina Sukhodolo
Department of Morphology and General Pathology, Siberian State Medical University, Tomsk 634050, Russia
Mikhail Belousov
Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk 634050, Russia
The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characterized previously. PS-SC was first isolated from the leaves extract of S. controversa and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of Staphylococcus aureus strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC > F-SC) at doses of 10−50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of S. controversa and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications.
