Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli

Abstract

Louise Hamborg et al. use a cell-based screen in E. coli to look for mutants of chymotrypsin inhibitor 2 which are either destabilized or stabilized, in an effort to identify a larger library of mutants which might improve stability prediction. Their work demonstrates how subtle conformational effects in proteins may modulate protein stability.