PRL/microRNA-183/IRS1 Pathway Regulates Milk Fat Metabolism in Cow Mammary Epithelial Cells

Peixin Jiao; Yuan Yuan; Meimei Zhang; Youran Sun; Chuanzi Wei; Xiaolai Xie; Yonggen Zhang; Sutian Wang; Zhi Chen; Xiaolong Wang

doi:10.3390/genes11020196

Genes (Feb 2020)

PRL/microRNA-183/IRS1 Pathway Regulates Milk Fat Metabolism in Cow Mammary Epithelial Cells

Peixin Jiao,
Yuan Yuan,
Meimei Zhang,
Youran Sun,
Chuanzi Wei,
Xiaolai Xie,
Yonggen Zhang,
Sutian Wang,
Zhi Chen,
Xiaolong Wang

Affiliations

Peixin Jiao: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
Yuan Yuan: School of Nursing, Yangzhou University, Yangzhou 225009, China
Meimei Zhang: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
Youran Sun: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
Chuanzi Wei: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
Xiaolai Xie: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
Yonggen Zhang: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
Sutian Wang: State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
Zhi Chen: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Xiaolong Wang: College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

DOI: https://doi.org/10.3390/genes11020196
Journal volume & issue: Vol. 11, no. 2
p. 196

Abstract

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The aim of the study was to understand the internal relationship between milk quality and lipid metabolism in cow mammary glands. A serial of studies was conducted to assess the molecular mechanism of PRL/microRNA-183/IRS1 (Insulin receptor substrate) pathway, which regulates milk fat metabolism in dairy cows. microRNA-183 (miR-183) was overexpressed and inhibited in cow mammary epithelial cells (CMECs), and its function was detected. The function of miR-183 in inhibiting milk fat metabolism was clarified by triglycerides (TAG), cholesterol and marker genes. There is a CpG island in the 5′-flanking promoter area of miR-183, which may inhibit the expression of miR-183 after methylation. Our results showed that prolactin (PRL) inhibited the expression of miR-183 by methylating the 5′ terminal CpG island of miR-183. The upstream regulation of PRL on miR-183 was demonstrated, and construction of the lipid metabolism regulation network of microRNA-183 and target gene IRS1 was performed. These results reveal the molecular mechanism of PRL/miR-183/IRS1 pathway regulating milk fat metabolism in dairy cows, thus providing an experimental basis for the improvement of milk quality.

Published in Genes

ISSN: 2073-4425 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://www.mdpi.com/journal/genes/

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