Comparison of the Effects of Native and Commercial Probiotic on Gluten Degradation

Abstract

Background and Objectives: The main treatment for celiac disease is a gluten-free diet. Gluten infection occurs in 55%-32% of these patients, which can cause symptoms in these patients. Many patients with celiac disease are dissatisfied with a gluten-free diet and are interested in a non-dietary alternative treatment. Methods: In this study, probiotic samples of local dairy (milk, yogurt, and cheese) from Iran were collected in sterile conditions and after enzymatic extraction using a lysis buffer and a sonication method for enzyme activity of prolyl endopeptidase and aminopeptidase N were evaluated. Results: The highest absorption rate for Z-Gly-Pro-4-nitroanilide substrate that performs the evaluation of prolyl endopeptidase enzyme, respectively, for strains C10-1 (L. pentosus), B6-1 (L. fermentum), C5-2 (L.paracasei), Y5 (L.paracasei) and C11-1 (L. pentosus) and in relation to Leu-p-NA which assesses aminopeptidase N The highest uptake was observed for strains K1 (L. helveticus), B8-2 (L. plantarum), Y5 (L.paracasei), P2-3 and P35 (L. plantarum), respectively. All of the above strains showed significantly different uptake than the negative control containing buffer and substrate (P<0.05). Finally, the 16s rRNA sequence of the isolated strains was registered in NCBI database. Conclusion: The results of the present study showed that number of native Iranian probiotics have effective activity of prolyl endopeptidase and aminopeptidase N which can be used as a selective combination for gluten degradation and to help celiac patients.

Keywords

• probiotics
• glutens
• celiac disease
• prolyl oligopeptidases
• aminopeptidase n