Compatibility of the entomopathogenic fungus Beauveria bassiana with the insecticides fipronil, pyriproxyfen and hexaflumuron

Abstract

The compatibility of Beauveria bassiana isolate DEBI 002 (Atashgah) with fipronil, pyriproxyfenand hexaflumuron insecticides was assayed. To determine the impact of the insecticides on thegermination of the fungal spore, different concentrations of the compounds were added to the culturemedium (malt agar). In order to calculate the mycelial growth in different treatments, each colonydiameter was measured and the spores were counted in the surface area to assess its sporulation. Theresults showed that pyriproxyfen at 1500 ppm and hexaflumuron at 80 ppm completely inhibited mycelialgrowth, while the inhibitory effect of fipronil at 1600 ppm remained at 76.6%. The inhibitory effect oflower concentrations of all three insecticides was between 10 and 20%. All tested insecticides inhibitedthe spore production between 80 to 100% at the highest concentrations without any significantdifferences. Pyriproxyfen at 400 ppm and hexaflumuron at all concentrations completely inhibited sporegermination, with significant difference, comparing with the rest of treatments. The results indicate thathexaflumuron has the highest inhibitory effect on the spore germination and is not recommended to beused simultaneously with B. bassiana against the insect pests.

Keywords

• beauveria bassiana
• compatibility
• fipronil
• hexaflumuron
• pyriproxyfen