INDUÇÃO DE CALOS EMBRIOGÊNICOS NAS CULTIVARES BRS ARARIPE E BRS SERIDÓ DO ALGODOEIRO

Abstract

The callus culture facilitates the study of factors involved in somatic embryogenesis, technique that allows the production of embryos in vitro from somatic tissue, making the propagation possible of elite plants and constituting an important tool in breeding programs. The objective was to analyze the influence of growth regulators on the formation of callus on cotton cultivars BRS Araripe and BRS Seridó. In the Laboratory of Tissue Culture of Embrapa Algodão, for the induction and proliferation of callus, hypocotyledonary segments were cultured on MS medium supplemented with phytoregulators NAA, KIN and 2,4-D. For the induction of embryogenic callus, it was used redifferentiation medium, with no phytoregulators. Data were analyzed according to the nonparametric chi-square test, being verified high number of potentially embryogenic callus, with a friable consistence and cream-greenish coloration. In the treatment with addition of NAA and KIN was observed the formation of embryogenic callus of BRS Seridó. The dependence of the genotype is a major problem for the low frequency of somatic embryogenesis in cotton that has been achieved in a few cultivars; it is necessary to study in other varieties for the definition of appropriate protocols in this process.