Sulfated polysaccharides of Turbinaria conoides dose-dependently mitigate oxidative stress by ameliorating antioxidants in isoproterenol induced myocardial injured rats: Evidence from histopathological study

Abstract

Objectives: In recent years, fucoidan is being marketed as a nutraceutical and food supplement because of its various pharmacological activity. Hence, we evaluated the cardioprotective activity of fucoidan extracted from Turbinaria conoides in isoproterenol induced myocardial injured rats. Methods: Wistar rats (180 ± 20 g) were divided into eight groups of six animals each and fucoidan was administered orally in three different doses (50, 100 and 150 mg/kg), for 14 days. At the end of this period, all the rats, except control group and fucoidan alone treated groups, were administered isoproterenol for two consecutive days to induce myocardial injury. After 48 h, rats were anesthetized, sacrificed and the levels of biochemical parameters were determined and histopathological analysis carried out. Results: Biochemical assessment of myocardial injury was done by measuring the activities of creatine kinase and lactate dehydrogenase, which were significantly elevated in the rats administered with isoproterenol. Further, pretreatment of fucoidan significantly normalized both endo and exogenous antioxidant defense system in isoproterenol induced myocardial injured rats. Therefore, fucoidan considerably reduced oxidative stress. In addition, histopathological findings were in line with biochemical findings. Conclusion: As a consequence, fucoidan possesses cardioprotective and antioxidative effect against isoproterenol induced myocardial injury in rats.

Keywords

• Isoproterenol
• Myocardial injury
• Antioxidants
• Oxidative stress
• Turbinaria conoides