Investigation of In Vitro Antioxidant and Antibacterial Potential of Silver Nanoparticles Obtained by Biosynthesis Using Beech Bark Extract
Corneliu Tanase,
Lavinia Berta,
Năstaca Alina Coman,
Ioana Roșca,
Adrian Man,
Felicia Toma,
Andrei Mocan,
László Jakab-Farkas,
Domokos Biró,
Anca Mare
Affiliations
Corneliu Tanase
Department of Pharmaceutical Botany, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, Târgu Mureș, 540139 Mureș, Romania
Lavinia Berta
Department of General and Inorganic Chemistry, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, Târgu Mureș, 540139 Mureș, Romania
Năstaca Alina Coman
Department of Pharmaceutical Botany, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, Târgu Mureș, 540139 Mureș, Romania
Ioana Roșca
Department of Pharmaceutical Botany, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, Târgu Mureș, 540139 Mureș, Romania
Adrian Man
Department of Microbiology, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, Târgu Mureș, 540139 Mureș, Romania
Felicia Toma
Department of Microbiology, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, Târgu Mureș, 540139 Mureș, Romania
Andrei Mocan
Department of Pharmaceutical Botany, ”Iuliu Hațieganu” University of Medicine and Pharmacy, 23 Gheorghe Marinescu Street, 400337 Cluj-Napoca, Romania
László Jakab-Farkas
Faculty of Technical and Human Sciences, Sapientia Hungarian University of Transylvania, 540485 Târgu Mureș, Romania
Domokos Biró
Faculty of Technical and Human Sciences, Sapientia Hungarian University of Transylvania, 540485 Târgu Mureș, Romania
Anca Mare
Department of Microbiology, “George Emil Palade” University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, Târgu Mureș, 540139 Mureș, Romania
Green synthesis is one of the rapid and best ways for silver nanoparticles (AgNP) synthesis. In the present study, synthesis and bioactivity of AgNPs has been demonstrated using water beech (Fagus sylvatica L.) bark extract. The physical and chemical factors such as time, metal ion solution, and pH, which play a vital role in the AgNPs synthesis, were assessed. The AgNPs were characterized by ultraviolet-visible (UV-Vis) spectrometry, Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM). Antioxidant and antimicrobial activity of the obtained AgNPs was evaluated. AgNPs were characterized by color change pattern, and the broad peak obtained at 420−475 nm with UV-Vis confirmed the synthesis of AgNPs. FT-IR results confirmed that phenols and proteins of beech bark extract are mainly responsible for capping and stabilization of synthesized AgNPs. TEM micrographs showed spherical or rarely polygonal and triangular particles with an average size of 32 nm at pH = 9, and 62 nm at pH = 4. Furthermore, synthesized AgNPs were found to exhibit antioxidant activity and have antibacterial effect against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa. These results indicate that bark extract of F. sylvatica L. is suitable for synthesizing stable AgNPs, which act as an excellent antimicrobial agent.
