Anti-Inflammatory Activity of <i>Panax notoginseng</i> Flower Saponins Quantified Using LC/MS/MS
Junchen Liu,
Yuehang Wu,
Wenrui Ma,
Hongyan Zhang,
Xianyao Meng,
Huirong Zhang,
Miaomiao Guo,
Xiao Ling,
Li Li
Affiliations
Junchen Liu
Beijing Key Lab of Plant Resource Research and Development, Institute of Cosmetic Regulatory Science, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China
Yuehang Wu
Beijing Key Lab of Plant Resource Research and Development, Institute of Cosmetic Regulatory Science, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China
Wenrui Ma
Beijing Key Lab of Plant Resource Research and Development, Institute of Cosmetic Regulatory Science, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China
Hongyan Zhang
Beijing Key Lab of Plant Resource Research and Development, Institute of Cosmetic Regulatory Science, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China
Xianyao Meng
Beijing Key Lab of Plant Resource Research and Development, Institute of Cosmetic Regulatory Science, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China
Huirong Zhang
Beijing Key Lab of Plant Resource Research and Development, Institute of Cosmetic Regulatory Science, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China
Miaomiao Guo
Beijing Key Lab of Plant Resource Research and Development, Institute of Cosmetic Regulatory Science, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China
Xiao Ling
Beijing Lan Divine Technology Co., Ltd., Beijing 100048, China
Li Li
Beijing Key Lab of Plant Resource Research and Development, Institute of Cosmetic Regulatory Science, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China
Panax notoginseng (Burk) F. H. Chen is a traditional Chinese medicinal and edible plant. However, Panax notoginseng flower (PNF) is rarely used. Therefore, the purpose of this study was to explore the main saponins and the anti-inflammatory bioactivity of PNF saponins (PNFS). We explored the regulation of cyclooxygenase 2 (COX–2), a key mediator of inflammatory pathways, in human keratinocyte cells treated with PNFS. A cell model of UVB-irradiation-induced inflammation was established to determine the influence of PNFS on inflammatory factors and their relationship with LL–37 expression. An enzyme-linked immunosorbent assay and Western blotting analysis were used to detect the production of inflammatory factors and LL37. Finally, liquid chromatography–tandem mass spectrometry was employed to quantify the main active components (ginsenosides Rb1, Rb2, Rb3, Rc, Rd, Re, Rg1, and notoginsenoside R1) in PNF. The results show that PNFS substantially inhibited COX–2 activity and downregulated the production of inflammatory factors, indicating that they can be used to reduce skin inflammation. PNFS also increased the expression of LL-37. The contents of ginsenosides Rb1, Rb2, Rb3, Rc, and Rd in PNF were much higher than those of Rg1, and notoginsenoside R1. This paper provides data in support of the application of PNF in cosmetics.
