COMPARATIVE EFFICACY OF RT-PCR, AGPT AND REVERSE PASSIVE HAEMAGGLUTINATION ASSAY FOR THE DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS IN BROILERS

Pakistan Veterinary Journal. 2006;26(4):167-170

 

Journal Homepage

Journal Title: Pakistan Veterinary Journal

ISSN: 0253-8318 (Print); 2074-7764 (Online)

Publisher: University of Agriculture, Faisalabad

Society/Institution: University of Agriculture, Faisalabad

LCC Subject Category: Agriculture: Animal culture: Veterinary medicine

Country of publisher: Pakistan

Language of fulltext: English

Full-text formats available: PDF

 

AUTHORS


M. S. MAHMOOD AND M. SIDDIQUE

EDITORIAL INFORMATION

Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 10 weeks

 

Abstract | Full Text

The reverse transcription polymerase chain reaction (RT-PCR), reverse passive haemagglutination assay (RPHA) and agar gel precipitation test (AGPT) were compared for the detection of infectious bursal disease virus (IBDV) in various organs of experimentally infected broilers. RT-PCR and RPHA required a time of 8 and 1 hour, respectively, whereas AGPT showed precipitation reaction at least after 24-48 hours of incubation. RT-PCR and RPHA were found sensitive to detect IBDV in tissues, whereas AGPT was less sensitive in the detection of IBDV. The RT-PCR was significantly more sensitive to detect IBDV as compared to RPHA, which was significantly more sensitive than AGPT in bursa of Fabricius, liver, kidneys, spleen and thymus samples. RPHA has given economical results for the detection of IBDV in samples from various organs and can be used in field conditions. However, RT-PCR was found to be the most sensitive test among the three tests compared in this study.