Comparison of the effects of platelet concentrates produced by high and low-speed centrifugation protocols on the healing of critical-size defects in rat calvaria: a microtomographic and histomorphometric study
Lucia Moitrel Pequeno da Silva,
Débora de Souza Ferreira Sávio,
Felipe Correa de Ávila,
Raphael Martini Vicente,
Gabriel Guerra David Reis,
Ricardo Junior Denardi,
Natacha Malu Miranda da Costa,
Pedro Henrique Felix Silva,
Carlos Fernando de Almeida Barros Mourão,
Richard J. Miron,
Michel Reis Messora
Affiliations
Lucia Moitrel Pequeno da Silva
Department of Oral and Maxillofacial Surgery and Periodontology – DCTBMF, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP
Débora de Souza Ferreira Sávio
Department of Morphology, Physiology, and Basic Pathology – DMFPB, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP
Felipe Correa de Ávila
Department of Morphology, Physiology, and Basic Pathology – DMFPB, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP
Raphael Martini Vicente
Department of Orthopedics and Anesthesiology, Ribeirão Preto Medical School, University of São Paulo – USP, Ribeirão Preto, São Paulo
Gabriel Guerra David Reis
Department of Oral and Maxillofacial Surgery and Periodontology – DCTBMF, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP
Ricardo Junior Denardi
Department of Oral and Maxillofacial Surgery and Periodontology – DCTBMF, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP
Natacha Malu Miranda da Costa
Department of Oral and Maxillofacial Surgery and Periodontology – DCTBMF, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP
Pedro Henrique Felix Silva
Department of Oral and Maxillofacial Surgery and Periodontology – DCTBMF, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP
Carlos Fernando de Almeida Barros Mourão
Clinical Research Unit, Antônio Pedro University Hospital, Fluminense Federal University, Rio de Janeiro
Richard J. Miron
Department of Periodontology, University of Bern, Bern
Michel Reis Messora
Department of Oral and Maxillofacial Surgery and Periodontology – DCTBMF, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP
The current study evaluated the healing of critical-size defects (CSD) created in rat calvaria treated with platelet concentrates produced by high-speed (Leukocyte- and Platelet-Rich Fibrin - L-PRF) and low-speed (Advanced Platelet-Rich Fibrin - A-PRF) protocols of centrifugation. Twenty-four rats were distributed into three groups: Control, L-PRF, and A-PRF. Five mm diameter CSD were created on the animals’ calvaria. The defects of the L-PRF and A-PRF groups were filled with 0.01 ml of L-PRF and A-PRF, respectively. The control group defects were filled with a blood clot only. All animals were euthanized on the 35th postoperative day. Histomorphometric and microtomographic analyses were then performed. The L-PRF and A-PRF groups had significantly higher bone volume and neoformed bone area than those of the control group and lowered bone porosity values (p < .05). No significant differences were observed between A-PRF and L-PRF groups for the analyzed parameters. Therefore, it can be concluded that i) L-PRF and A-PRF potentiated the healing of CSD in rat calvaria; ii) high and low-speed centrifugation protocols did not produce PRF matrices with different biological impacts on the amount of bone neoformation.
