EFFECT OF MULTIPROBIOTIC «SYMBITER ACIDOPHILIC» USED AGAINST THE BACKGROUND OF THE COURSE OF INJECTIONS OF CEFTRIAXONE ANTIBIOTIC ON PERITONEAL MACROPHAGE FUNCTIONAL ACTIVITY IN RAT

Abstract

The aim of the work was to investigate the effect of probiotic preparation “Symbiter acidophilic” used against the background of the course of injection of ceftriaxone antibiotic on macrophage functional activity in Wistar rats. Methods. Metabolic state and functional polarization of peritoneal macrophages were characterized by measurement of their adhesion to the cultural plastic, spontaneous and stimulated oxidative metabolism, arginase activity and NO production. Results. The course of injection of the preparation “Symbiter acidophilic” against the background of the course of injection ceftriaxone antibiotic, as well as administration of both preparations separately, was associated with the stimulation of macrophage oxidative metabolism accompanied by M1-polarization of arginine metabolism. All together it indicates proinflammatory polarization of activated mononuclear phagocytes. Conclusion. Administration of the preparation “Symbiter acidophilic” against the background of the course of injection of ceftriaxone antibiotic promoted the forming of neutral balance of arginine metabolism in peritoneal macrophages that can be considered as a sign of inhibition of antibiotic-induced inflammatory process.

Keywords

• пробіотик
• антибіотик
• перитонеальні макрофаги
• кисеньзалежний метаболізм
• метаболізм аргініна