This paper reports a calorimetric micro-sensor designed for aerodynamic applications. Measuring both the amplitude and the sign of the wall shear stress at small length-scale and high frequencies, the micro-sensor is particularly suited for flow separation detection and flow control. The micro-sensor was calibrated in static and dynamic in a turbulent boundary layer wind tunnel. Several micro-sensors were embedded in various configurations for measuring the shear stress and detecting flow separation. Specially, one was embedded inside an actuator slot for in situ measurements and twelve, associated with miniaturized electronics, were implemented on a flap model for active flow control experiments.