Utilization of Rice Straw (Oryza sativa Linn) Agricultural Waste as Substrate for Poly(3-Hydroxybutyrate) Production Using Pseudomonas aeruginosa

Abstract

Utilization of rice straw (Oryza sativa Linn) as a raw material in the production of poly(3-hydroxybutyrate), P(3HB), biopolymer by fermentation using Pseudomonas aeruginosa has been conducted. Rice straw stew and microcrystalline cellulose obtained from rice straw were used as carbon source for bacterial growth at various concentrations. The content of P(3HB) in the biomass was determined by gas chromatography using the RTX-1 column and the FID detector. Results showed that the best condition in producing P(3HB) was using 1% microcrystalline cellulose as carbon source and duration of fermentation of 48 hours. The biomass and P(3HB) obtained from MCC were 982 mg/100 mL and 17.19% w/w, respectively. While using rice straw stew were 269 mg/100 mL and 1.73%, respectively. It can be concluded that the highest P(3HB) produced was found using microcrystalline cellulose obtained from rice straw waste as a carbon source.

Keywords

• rice straw
• poly(3-hydroxybutyrate)
• pseudomonas aeruginosa
• microcrystalline cellulose