Cytological characterization of Jatropha curcas callus in different periods of cultivation

Abstract

The aimed was characterization and determining the time for Jatropha curcas callus subculture. The pattern of J. curcascallus development is sigmoidal with 5 distinct phases, and transplantation should be performed at the end of the deceleration phase(approximately day 28). During callus development, it was observed that at the onset of growth in the exponential phase the cells wereinternally disorganized; in the linear phase, respiratory metabolism was resumed through reorganization of the first mitochondria;and by the mid-deceleration phase, the cells were entirely active, and several organelles were detected. This organizational status wasmaintained throughout the stationary phase during which somatic pre-embryos were identified. At the end of the stationary phase, intracellulardisruption began, and the cells entered senescence, which characterized the decline phase for the J. curcas calli growth curve.

Keywords

• In vitro
• kinetics and ultrastructure.