DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE HPLC-UV METHOD TO DETERMINE CAFFEINE IN GUARANA (Paullinia cupana) FOOD SUPPLEMENTS

Abstract

Caffeine and guarana are safe foods according to the American (FDA) and Brazilian (ANVISA) health agencies. However, data regarding the composition, quality, and safety of guarana-based food supplements sold in Brazil are limited. Most of the methods used for quantification of caffeine and other guarana chemical markers are based on complex extraction techniques as well as on gradient elution and do not evaluate the matrix effect nor the uncertainty estimation measurement. A simple and selective method for caffeine detection has been developed and validated using HPLC-UV. It shows linearity between 1 and 10 µg mL-1, has a significant matrix effect (p 0.005).

Keywords

• bioactive substances
• food supplements
• high performance liquid chromatography
• methylxanthines
• quality control