Expression of nephronectin is inhibited by oncostatin M via both JAK/STAT and MAPK pathways

Tamaki Kurosawa; Atsushi Yamada; Masamichi Takami; Dai Suzuki; Yoshiro Saito; Katsuhiro Hiranuma; Takuya Enomoto; Naoko Morimura; Matsuo Yamamoto; Takehiko Iijima; Tatsuo Shirota; Hiroyuki Itabe; Ryutaro Kamijo

doi:10.1016/j.fob.2015.04.001

FEBS Open Bio (Jan 2015)

Expression of nephronectin is inhibited by oncostatin M via both JAK/STAT and MAPK pathways

Tamaki Kurosawa,
Atsushi Yamada,
Masamichi Takami,
Dai Suzuki,
Yoshiro Saito,
Katsuhiro Hiranuma,
Takuya Enomoto,
Naoko Morimura,
Matsuo Yamamoto,
Takehiko Iijima,
Tatsuo Shirota,
Hiroyuki Itabe,
Ryutaro Kamijo

Affiliations

Tamaki Kurosawa: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan
Atsushi Yamada: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan
Masamichi Takami: Department of Pharmacology, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan
Dai Suzuki: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan
Yoshiro Saito: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan
Katsuhiro Hiranuma: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan
Takuya Enomoto: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan
Naoko Morimura: Brain Science Laboratory, The Research Organization of Science and Technology, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan
Matsuo Yamamoto: Department of Periodontology, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan
Takehiko Iijima: Department of Perioperative Medicine Division of Anesthesiology, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan
Tatsuo Shirota: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ohta, Tokyo 145-8515, Japan
Hiroyuki Itabe: Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Shinagawa, Tokyo 142-8555, Japan
Ryutaro Kamijo: Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan

DOI: https://doi.org/10.1016/j.fob.2015.04.001
Journal volume & issue: Vol. 5, no. 1
pp. 303 – 307

Abstract

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Nephronectin (Npnt), also called POEM, is an extracellular matrix protein considered to play critical roles as an adhesion molecule in the development and functions of various tissues, such as the kidneys, liver, and bones. In the present study, we examined the molecular mechanism ofNpnt gene expression and found that oncostatin M (OSM) strongly inhibitedNpnt mRNA expression in MC3T3‐E1 cells from a mouse osteoblastic cell line. OSM also induced a decrease inNpnt expression in both time‐ and dose‐dependent manners via both the JAK/STAT and MAPK pathways. In addition, OSM‐induced inhibition of osteoblast differentiation was recovered by over‐expression ofNpnt. These results suggest that OSM inhibitsNpnt expression via the JAK/STAT and MAPK pathways, while down‐regulation ofNpnt by OSM influences inhibition of osteoblast differentiation.

Published in FEBS Open Bio

ISSN: 2211-5463 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://febs.onlinelibrary.wiley.com/journal/22115463

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