Nature Communications (Jan 2019)

Engineering of CRISPR-Cas12b for human genome editing

  • Jonathan Strecker,
  • Sara Jones,
  • Balwina Koopal,
  • Jonathan Schmid-Burgk,
  • Bernd Zetsche,
  • Linyi Gao,
  • Kira S. Makarova,
  • Eugene V. Koonin,
  • Feng Zhang

DOI
https://doi.org/10.1038/s41467-018-08224-4
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 8

Abstract

Read online

The Cas12b family of CRISPR nucleases has been underutilized in mammalian cells due to the high temperature requirement of known members. Here the authors engineer BhCas12b to overcome this limitation for robust and specific genome editing applications in human cells.